DETERMINATION OF ANIMAL SPECIES
[TIERARTENBESTIMMUNG]
EPTIS factsheet 154131 | Last revision 2021-10-29 | URL: https://www.eptis.bam.de/pts154131 https://www.eptis.bam.de/pts154131
| PT provider | ||||||||||||||||
| PT provider | DRRR - Deutsches Referenzbüro für Ringversuche und Referenzmaterialien GmbH DRRR - Deutsches Referenzbüro für Ringversuche und Referenzmaterialien GmbH | |||||||||||||||
| Based in | Germany | |||||||||||||||
| Language(s) | German/English | |||||||||||||||
| Remarks | Nowadays the demands for most sensitive animal species detection in particular of pork is becoming of increasing interest. The molecular biological real-time PCR method, based on specific DNA amplification detects highly specific DNA and DNA fragments of the relevant species. The sensitivity is based on the detection system and the food matrix and processing grade. Determining Ct values of the real-time PCR run, relative quantification is possible. However systems are calibrated on raw muscle tissue. In this ring trial stable gelatin is used as a matrix for qualitative detection of low amounts of present porcine DNA. Based on the former successful profiency test RVEP 150204 whereas porcine DNA (fragments) were specifically identified in gelatin for human consumption, the new profiency test will include the sensitive detection of pork (sus scrofa) and optionally also of bovine (bos taurus). The PT is designed to use DNA methods (preferentially qPCR), but other tests might be used as well. The profiency test will consist of a sensitive qualitative detection of traces of DNA (fragments). Even so the achieved ct values will indicate how strong the results and therefore sensitivity of different systems are. A real quantification will not be possible in this matrix. | |||||||||||||||
| Keywords | ||||||||||||||||
| Product groups |
Animal test material
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| Testing fields |
Analytical chemistry
Veterinary analysis |
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| Technical details | ||||||||||||||||
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| Aims of the PT scheme | ||||||||||||||||
| Target group of participants | all laboratories | |||||||||||||||
| Linked to specific legislation / standards | ||||||||||||||||
| Additional, subsidiary aims | validation of testing methods | |||||||||||||||
| Number of participants | ||||||||||||||||
| Accredited or otherwise reviewed by a 3rd party |
Accredited by A2LA (USA) and DAkkS (Germany) on the basis of for part of the parameters |
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| Operation is commissioned / requested by | ||||||||||||||||
| Fees and frequency | ||||||||||||||||
| Participation fee | 331 EUR | |||||||||||||||
| Regularly operated | Yes (several times p.a., dep. on sample and parameters) | |||||||||||||||
| Year of first operation | since 2015, 2020, 2021 | |||||||||||||||
| Contact details of the PT provider | ||||||||||||||||
| Provider | Contact person | |||||||||||||||
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DRRR - Deutsches Referenzbüro für Ringversuche und Referenzmaterialien GmbH
Reinhartser Straße 31 87437 Kempten Germany Phone: +49 (0) 831/960 8780 Fax: +49 (0) 831/960 87899 Web: http://www.drrr.de http://www.drrr.de |
Dr. Ulrich Leist
Phone: +49 (0) 831/960 87878 Fax: +49 (0) 831/960 87899 Email: info@drrr.de info@drrr.de |
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If you find any mistakes please contact the responsible EPTIS coordinator in Germany,
Mr Johannes van de Kreeke.
Mr Johannes van de Kreeke.