DETERMINATION OF ANIMAL SPECIES
[TIERARTENBESTIMMUNG]
EPTIS Ringversuch Nr. 154131 | Letze Änderung 2021-10-29 | URL: https://www.eptis.bam.de/pts154131 https://www.eptis.bam.de/pts154131
| Name des Anbieters | ||||||||||||||||
| Name des Anbieters | DRRR - Deutsches Referenzbüro für Ringversuche und Referenzmaterialien GmbH DRRR - Deutsches Referenzbüro für Ringversuche und Referenzmaterialien GmbH | |||||||||||||||
| Ansässig in | Germany | |||||||||||||||
| Sprache(n) | German/English | |||||||||||||||
| Anmerkungen | Nowadays the demands for most sensitive animal species detection in particular of pork is becoming of increasing interest. The molecular biological real-time PCR method, based on specific DNA amplification detects highly specific DNA and DNA fragments of the relevant species. The sensitivity is based on the detection system and the food matrix and processing grade. Determining Ct values of the real-time PCR run, relative quantification is possible. However systems are calibrated on raw muscle tissue. In this ring trial stable gelatin is used as a matrix for qualitative detection of low amounts of present porcine DNA. Based on the former successful profiency test RVEP 150204 whereas porcine DNA (fragments) were specifically identified in gelatin for human consumption, the new profiency test will include the sensitive detection of pork (sus scrofa) and optionally also of bovine (bos taurus). The PT is designed to use DNA methods (preferentially qPCR), but other tests might be used as well. The profiency test will consist of a sensitive qualitative detection of traces of DNA (fragments). Even so the achieved ct values will indicate how strong the results and therefore sensitivity of different systems are. A real quantification will not be possible in this matrix. | |||||||||||||||
| Klassifizierung | ||||||||||||||||
| Produktgruppen |
Animal test material
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| Prüfgebiete |
Analytical chemistry
Veterinary analysis |
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| Technische Einzelheiten | ||||||||||||||||
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| Ringversuchsziele | ||||||||||||||||
| Zielgruppe des Ringversuchs | all laboratories | |||||||||||||||
| Relevante Rechtsvorschriften oder Normen | ||||||||||||||||
| Weitere Ziele | validation of testing methods | |||||||||||||||
| Teilnehmerzahl | ||||||||||||||||
| Anbieter unabhängig überprüft (akkreditiert, benannt, anerkannt) |
Akkreditiert durch A2LA (USA) and DAkkS (Germany) auf der Grundlage von nicht für alle Parameter |
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| Die Durchführung des Ringversuchs ist in Auftrag gegeben durch | ||||||||||||||||
| Kosten | ||||||||||||||||
| Teilnahmegebühr | 331 EUR | |||||||||||||||
| Regelmässig durchgeführt | Ja (several times p.a., dep. on sample and parameters) | |||||||||||||||
| Der Ringversuch wird durchgeführt seit | since 2015, 2020, 2021 | |||||||||||||||
| Kontaktdaten des Anbieters | ||||||||||||||||
| Anbieter | Kontaktperson | |||||||||||||||
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DRRR - Deutsches Referenzbüro für Ringversuche und Referenzmaterialien GmbH
Reinhartser Straße 31 87437 Kempten Germany Telefon: +49 (0) 831/960 8780 Fax: +49 (0) 831/960 87899 Web: http://www.drrr.de http://www.drrr.de |
Dr. Ulrich Leist
Telefon: +49 (0) 831/960 87878 Fax: +49 (0) 831/960 87899 E-Mail: info@drrr.de info@drrr.de |
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