EPTIS
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DETERMINATION OF ANIMAL SPECIES

[TIERARTENBESTIMMUNG]

EPTIS factsheet 154131 | Last revision 2020-10-21 | URL: https://www.eptis.bam.de/pts154131 https://www.eptis.bam.de/pts154131

PT provider
PT provider DRRR - Deutsches Referenzbüro für Ringversuche und Referenzmaterialien GmbH DRRR - Deutsches Referenzbüro für Ringversuche und Referenzmaterialien GmbH
Based in Germany
Language(s) German/English
Remarks Nowadays the demands for most sensitive animal species detection in particular of pork is becoming of increasing interest. The molecular biological real-time PCR method, based on specific DNA amplification detects highly specific DNA and DNA fragments of the relevant species. The sensitivity is based on the detection system and the food matrix and processing grade. Determining Ct values of the real-time PCR run, relative quantification is possible. However systems are calibrated on raw muscle tissue. In this ring trial stable gelatin is used as a matrix for qualitative detection of low amounts of present porcine DNA. Based on the former successful profiency test RVEP 150204 whereas porcine DNA (fragments) were specifically identified in gelatin for human consumption, the new profiency test will include the sensitive detection of pork (sus scrofa) and optionally also of bovine (bos taurus). The PT is designed to use DNA methods (preferentially qPCR), but other tests might be used as well. The profiency test will consist of a sensitive qualitative detection of traces of DNA (fragments). Even so the achieved ct values will indicate how strong the results and therefore sensitivity of different systems are. A real quantification will not be possible in this matrix.
Keywords
Product groups Animal test material
Testing fields Analytical chemistry
Veterinary analysis
Technical details
Test item Tested property Testing method
Gelatine containing porcine DNA Qualitative detection of low amounts of porcine DNA in highly processed food matrix (gelatin) -
Meat Determination of animal species (horse or swine) -
Porcine DNA in Candy Qualitative detection of low amounts of porcine (sus scrofa) DNA in candy (gummy candy) -
Detection of the animal species in milk animal species qualitative -
Aims of the PT scheme
Target group of participants all laboratories
Linked to specific legislation / standards
Additional, subsidiary aims validation of testing methods
Number of participants
Accredited or otherwise reviewed by a 3rd party

Accredited by A2LA (USA) and DAkkS (Germany) on the basis of for part of the parameters

Operation is commissioned / requested by
Fees and frequency
Participation fee 331 EUR
Regularly operated Yes (several times p.a., dep. on sample and parameters)
Year of first operation since 2015, 2020, 2021
Contact details of the PT provider
Provider Contact person
DRRR - Deutsches Referenzbüro für Ringversuche und Referenzmaterialien GmbH
Bodmanstraße 4
87435 Kempten
Germany

Phone: +49 (0) 831/960 8780
Fax: +49 (0) 831/960 87899
Web: http://www.drrr.de http://www.drrr.de
Dr. Ulrich Leist
Phone: +49 (0) 831/960 87878
Fax: +49 (0) 831/960 87899
Email: info@drrr.de info@drrr.de
If you find any mistakes please contact the responsible EPTIS coordinator in Germany, Mr Johannes van de Kreeke. Mr Johannes van de Kreeke.
Any questions or problems? Please contact us at eptis@bam.de.
Application version: 1.21