EPTIS
BAM Logo

Interlaboratory study for effect-based in vitro methods to detect estrogenic activity (estrogenic effects)

[Ringversuch für wirkungsbezogene in vitro Verfahren zum Nachweis der estrogenen Aktivität (Estrogene Effekte)]

EPTIS factsheet 809344 | Last revision 2020-12-22 | URL: https://www.eptis.bam.de/pts809344 https://www.eptis.bam.de/pts809344

PT provider
PT provider QuoData Quality and Statistics QuoData Quality and Statistics
Based in Germany
Language(s) English
Remarks The interlab comparison exercise provides valuable information on the comparability and reproducibility of the in vitro methods used.
Keywords
Product groups Cosmetics
Food and drink
Water
Testing fields Analytical chemistry
Environmental protection
Technical details
Test item Tested property Testing method
Waste water Estradiol equivalents (EEQ) / Estradiol equivalent concentration Effect based in vitro methods / bioassays such as Yeast estrogen screen (YES), Arxula yeast estrogen screen (A-YES), human-cell based reporter gene assays (CALUX, Chemical Activated LUciferase gene eXpression) or cell proliferation assays
Lowest ineffective dilution (LID) Effect based in vitro methods / bioassays such as Yeast estrogen screen (YES), Arxula yeast estrogen screen (A-YES), human-cell based reporter gene assays (CALUX, Chemical Activated LUciferase gene eXpression) or cell proliferation assays
Extract of waste water or surface water Estradiol equivalents (EEQ) / Estradiol equivalent concentration Effect based in vitro methods / bioassays such as Yeast estrogen screen (YES), Arxula yeast estrogen screen (A-YES), human-cell based reporter gene assays (CALUX, Chemical Activated LUciferase gene eXpression) or cell proliferation assays
Surface water Estradiol equivalents (EEQ) / Estradiol equivalent concentration Effect based in vitro methods / bioassays such as Yeast estrogen screen (YES), Arxula yeast estrogen screen (A-YES), human-cell based reporter gene assays (CALUX, Chemical Activated LUciferase gene eXpression) or cell proliferation assays
Lowest ineffective dilution (LID) Effect based in vitro methods / bioassays such as Yeast estrogen screen (YES), Arxula yeast estrogen screen (A-YES), human-cell based reporter gene assays (CALUX, Chemical Activated LUciferase gene eXpression) or cell proliferation assays
Extract of personal care product Estradiol equivalents (EEQ) / Estradiol equivalent concentration Effect based in vitro methods / bioassays such as Yeast estrogen screen (YES), Arxula yeast estrogen screen (A-YES), human-cell based reporter gene assays (CALUX, Chemical Activated LUciferase gene eXpression) or cell proliferation assays
Aims of the PT scheme
Target group of participants Accredited and non-accredited laboratories
Linked to specific legislation / standards Related to the following standards, but not limited to these: ISO 19040 Part 1 (Determination of the estrogenic potential of water and waste water - Yeast estrogen screen (Saccharomyces cerevisiae)) ISO 19030 Part 2 (Determination of the estrogenic potential of water and waste water - Yeast estrogen screen (A-YES, Arxula adeninivorans)) ISO 19040 Part 3 (Determination of the estrogenic potential of water and waste water - In vitro human cell-based reporter gene assay)
Additional, subsidiary aims
Number of participants > 25
Accredited or otherwise reviewed by a 3rd party
Operation is commissioned / requested by
Fees and frequency
Participation fee 300 € net per method
Regularly operated Yes (once per year)
Year of first operation 2021
Contact details of the PT provider
Provider Contact person
QuoData Quality and Statistics
Prellerstraße 14
01309 Dresden
Germany

Phone: +49 (0)3 51 - 4028867 0
Fax: +49 (0)3 51 - 4028867 19
Web: https://www.quodata.de/en https://www.quodata.de/en
Herr Conny Lerche
Phone: 0351-4028867-41
Fax: 0351-4028867-19
Email: lerche@quodata.de lerche@quodata.de
If you find any mistakes please contact the responsible EPTIS coordinator in Germany, Mr Johannes van de Kreeke. Mr Johannes van de Kreeke.
Any questions or problems? Please contact us at eptis@bam.de.
Application version: 1.21